Plate 25 μL of cells in. The adapterResults summary for HTRF IP1 standard curve. Read & analyse. Dual-Luciferase® (DLR™) gene assays. EnVision Nexus ultimode Plate Reader 2 Benefits • Provides the speed and sensitivity to boost your lab’s productivity with ultra-high throughput • Freely configurable and upgradable to adapt to your needs today and tomorrow • Fully automatable for walkaway convenience • Certified for use with HTRF® technology Key featuresBMG LABTECH plate readers certified for LanthaScreen detection include the PHERAstar FSX, CLARIOstar Plus and VANTAstar. Table 1. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. Seal the plate and incubate 3 hours at RT . FOR HTRF CERTIFIED READER For more. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. ratio, high sensitivity, wide dynamic range, superior stability, and excellent flexibility. Remove the plate seal and read on an HTRF compatible reader. The 2 plate protocol involves culturing cells in a 96-well plate before lysis then transferring lysates to a 384-well low volume detection plate before adding Phospho-ERK (Thr202/Tyr204) HTRF detection reagents. These are the different kinetic modes on BMG LABTECH microplate readers. The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. FOR HTRF CERTIFIED READER. protocol with suspension cells or with a. 9. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. Finding a plate reader that offers compatibility with microplates sold by multiple leading vendors can protect both the long-term cost and the performance of assays. 5 nm filter (PerkinElmer 2100-5110) were used. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, AAS System, and dedicated AlphaScreen ® and. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. (665 nm and 620 nm) on a compatible HTRF ® reader. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. FOR HTRF CERTIFIED READER For. Make sure the setup for Eu3+ Cryptate is used. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. • HTRF®-Certified Reader **. FOR HTRF CERTIFIED READER. For HTRF certified readerThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. PerkinElmer. As shown in Fig. • HTRF plate validated (QC with reference HTRF assays) • Low to high volumes (10 µL to 350 µL) • Can be used with all HTRF compatible readers HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. GENERAL LAB WORK USING HTRF KIT 1 Plate 3 mL of cells in 6-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Incubate overnight at RT. Download Setup. CLARIOstar is equipped with a specific optical device which enables the measurement of. Set up your reader for Eu3+ Cryptate and read the fluorescence emission at two different wavelengths (665nm and 620nm) on a compatible HTRF® reader**. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. It features a modular design that allows users to add or change detection cartridges according to their needs. These plates can be read on all HTRF certified readers, thus making their implementation in. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. The VICTOR Nivo is a HTRF certified microplate reader. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-3 (Ser473) by HTRF. Customer Transformation Hub. Dual monochromators for assay flexibility With. Read & analyse. Lyse cells Read on an HTRF compatible reader . Incubate overnight at RT. no. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. The Infinite 200 PRO is an easy-to-use multimode plate reader family that offers affordable, high performance detection solutions, referenced in more than 1800 peer reviewed. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Make sure the setup for Tb Cryptate is used. Step 5 Remove the plate sealer and read on an HTRF® compatible reader The blank control is used to check the Cryptate signal at 620 nm. The samples were each placed in a well of a 24-well plate, covered with 1 ml of culture medium, and incubated for a total of 17 days. For more information about HTRF. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). VICTOR Nivo Multimode Plate Reader • Compact and lightweight • High performance for routine applications, including biochemical and cell-based assays • All major detection. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3 Ordering information VWR cat. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Taking these considerations into account, Cisbio’s 96w low volume and PKI’s 96w & 384w microplates have been carefully designed to provide the best signal detection environment possible to all HTRF assays. FOR HTRF CERTIFIED READER For. Incubate overnight at RT. HTRF is compatible with a large range of available microplate readers. Read & analyse. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. There are multiple factors to consider including: detection type, additional. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. Plate 25 μL of cells in. FOR HTRF CERTIFIED READER. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. Incubate 4h at RT. Incubate overnight at RT. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of phospho- CHK2 (Thr 68) by HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 5 hours before 3 more washing steps. FOR HTRF CERTIFIED READER. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. Figure 3. Read & analyse. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. CLARIOstar is equipped with a specific optical device which enables the measurement of. Plate 25 μL of cells in. and Canaa -8-963-4567. Seal the plate and incubate 1 hour at RT : Remove the plate sealer and read on an HTRF® compatible reader . Plate 25 μL of cells in 96 half-well plate in your appropriate medium. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. 1. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. For more ® information about HTRF compatible readers and for set-up recommendations, please. Lysates are then transferred to the assay plate for the detection of total-RET by HTRF. These plates can be read on all HTRF certified readers, thus making their implementation in. The system provides even higher sensitivity for time-resolved fluorescence (TRF) applications. It can also be further streamlined to a one-. The plate map included a low and a high level calibrator, conjugate blanks, and a specific control to assess the counting rate at 620 nm (i. $352. Incubate overnight at RT. For more ® information about HTRF compatible readers and for set-up recommendations, please. For more information about HTRF. For more information about HTRF. Read & analyse. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. CLARIOstar is equipped with a specific optical device which enables the measurement of. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Suggested plate map: 1: 2 3: A: 14 μL Std 0: 3 μL IP1 d2 Reagent 3 μL IP1 Tb Cryptate Antibody• HTRF®-Certified Reader. HTRF is compatible with a large range of available microplate readers. One plate carrier adapter is provided with the instrument. If not, our scientific team will help you choose the instrument that best suits your needs and budget. FOR HTRF CERTIFIED READER. overnight at 4 C [Prostaglandin E2] pg/ml. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. FOR HTRF CERTIFIED READERFOR HTRF CERTIFIED READER. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. This DLReady-certified microplate reader can also come equipped with Simultaneous Dual Emission for BRET applications and can be further upgraded to a multi-mode reader. These plates can be read on all HTRF certified readers, thus making their implementation in. Incubate 4h at RT. Preconfigured HTRF. Plus . The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. For more ® information about HTRF compatible readers and for set-up recommendations. For more information about HTRF ®• HTRF®-Certified Reader. For more information about HTRF. The dual measurement. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes• HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. Remove the plate sealer and read on an HTRF® compatible reader . 96-well culture plate 384SV detection plate- Add . Both readers demonstrated their ability to perform the cAMP G s HiRange assay with results well within Cisbio’s required. • Our fastest benchtop plate reader – uses two detectors to enable simultaneous, dual-wavelength reading • Well established for laser-based Alpha and TRF technologies, as well as ultrasensitive luminescence • Time-resolved fluorescence certified for use with HTRF ® technology • Software that can facilitate 21 CFR Part 11 compliance for Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Whatever the plate type, assay volume should match well volume as closely as possible so that focalization of the reader excitation beam gives maximum energy delivery (Fig. Make sure the setup for Tb Cryptate is used. With its ease of use software and remote access from almost any device, it is ideal for multiuser environments. -Certified Reader** *Assay volumes can be adjusted proportionally to run the assay in 96- or 1536-well microplates. Our assay development platform relies on PHERAstar readers for absorbance, fluorescence, luminescence, and homogeneous time resolved. Incubate 4h at RT. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. + 4 µL HTRF pre-mixed antibodies. Cover the plate with a plate sealer. HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Signals are detected at two different wavelengths (typically 620 nm and 665 nm), which are used to. The Negative control is used to check the non-specific signal. After defining the objectives and their locations in Gen5: 1. For more information about HTRF ®Highly compatible plates for HTRF. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. The EnVision XCite Plate Reader provides exceptional sensitivity and speed with minimal downtime - backed by our global network of application scientists and engineers. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. and TR-FRET, for example to detect PerkinElmer’s DELFIA, HTRF and LANCE assay technologies Time resolved fluorescence certified for use with HTRF. For a list of HTRF-compatible readers and set-up recommendations, please visit . Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. These plates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy Provider Format Part Numbers Recommended volume Type; Cisbio: 96-w low volume: 6PL96005 – 66PL96025 – 66PL96100: 20-30 µL:HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. For more information about HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Reagents were dispensed following Cisbio’s ligand binding protocol (Figure 3). Thanks to HTRF signal stability, the assay plate can be used to check several instruments, or different reader. HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. The new Molecular Devices instrument comes with a standard 1-year depot warranty service. Read & analyse. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Lysates are then transferred to ® the assay plate for the detection of Total EGFR L858R by HTRF reagents. You can count on our plate readers to bring you the performance, reliability, consistent results, and the best user experience available—across a multitude of applications and a range of detection options. Due to the chemical and photophysical. For a list of HTRF-compatible readers and set-up recommendations, please visit . The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. MATERIALS PROVIDED: KIT . For more information about HTRF. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. As shown in Fig. The donor and acceptor can be used to label a variety of biomolecules, for. Optimization of the reader for the plate dimensions clearly improved the results as seen by the uniform signal (b), a lowered %CV of 2. Note that any dilution or improper use of d2 and cryptate Tb-detection reagents will impair the assay quality. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Detection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Figure 1: Principle of HTRF Prostaglandin E2 competitive assay. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Make sure the setup for Tb Cryptate is used. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Some vendors hold the entire market share for not only a. Incubate 2 h at RT. FOR HTRF CERTIFIED READER For more ® information about HTRF compatible readers and for set-up recommendations, please visit our. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Partnerships for delivering turn-key solutions. • HTRF®-Certified Reader **. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. This protocol gives the cells viability and confluence to be monitored. 96-well culture plateFOR HTRF CERTIFIED READER. Better sensitivity provides better statistic, less variability among replicates, and a larger assay window. EM1 616/10 nm EM2 665/10 nm Certified for use with Cisbio’s HTRF Europium Cryptate reagents. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Make sure the setup for Eu3+ Cryptate is used. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Your lab might even have one already. Results for HTRF Reader Control Kit with black and white microplates. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. In this case the Z´(pronounced Z prime) value comes to mind. Lysates are then transferred to the assay plate for the detection of Total Cofilin by HTRF® reagents. HTRF® CertifiedCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. HTRF combines time-resolved fresh detection with FRET and is mainly used for drug screening in high-throughput. At Cisbio we provide microplates that help you discover better. If not, our scientific team will help you choose the instrument that best suits your needs and budget. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. PerkinElmer’s 96w low volume, 96w and 384w microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. 5. PROTOCOL AT A GLANCE. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Not for use in diagnostic procedures. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. PURCHASE SEPARATELY . VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the market with all major detection technologies including Alpha and certification for HTRF® technology plus software designed for. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. If not, our scientific team will help you choose the instrument that best suits your needs and budget. HTRF is a proprietary time-resolved fluoresce nce technology that overcomesCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. FOR HTRF CERTIFIED READER. Its. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. • ®HTRF -Certified Reader(5) (1) IBMX is the most commonly used phosphodiesterase Pan-inhibitor. Plate Type and Read Area should be selected based on the microplate used and wells to be read. FOR HTRF CERTIFIED READER. Catalog No. If not, our scientific team will help you choose the instrument that best suits your needs and budget. + 4 µL HTRF ® pre-mixed antibo dies 2. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. Incubate 4h at RT. A typical HTRF assay uses a europium cryptate as the donor, and the organic fluorophore d2 as the acceptor. (HTRF) • Certified for detection of Cisbio’s HTRF assays • GPCR assays (cAMP cell based, membrane based). Incubate 4h at RT. For more information about HTRF. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. Lysates are then transferred to the assay plate for the detection of Phospho-AKT-2 (Ser473) by HTRF. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. Read & analyse. Make sure the plate reader is turnedon and then open the MikroWin software on the computer. As shown in Fig. Plate cells Stimulate cells 3. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. FOR HTRF CERTIFIED READER. 96-well culture plateThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The SpectraMax® M5e includes htrf certification, and both the M5 and M5e are lanthascreen certified. FOR HTRF CERTIFIED READER. For a list of HTRF-compatible readers and set-up recommendations, please visit . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of total-CREB by HTRF. FOR HTRF CERTIFIED READER. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. Choosing the right microplate reader ensures you’ll get an optimal readout. Step 1 Step 2 Step 3 Step 4 Step 5. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Advanced Phospho-ERK (Thr202/Tyr204) 2-plate assay protocol. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . Revvity. TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. ® Detection of Progesterone Receptor with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Unlike fluorescence intensity which focuses on the quantification of emission intensity at a specific wavelength and neglects its polarization, fluorescence polarization specifically analyses as output the emission intensity of. No washing steps are required. It can also be further streamlinedFOR HTRF CERTIFIED READER. The readers detect light signals that result from biological, chemical, biochemical, or physical reactions. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The VICTOR Nivo. FOR HTRF CERTIFIED READER culture plate to a 384-well small volume white plate. NanoQuant Plate™ allows rapid, straightforward measurement of 16 x 2 µl samples simultaneously without calibration. Specialized filters allow for IR fluorescence. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. For the standard curve described, all wells of the microplate are read. Microplate reader assays requiring infrared (IR) or. For more information about HTRF. The ratio of the two fluorescence TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: + 10 µL lysis buffer ONE-PLATE ASSAY PROTOCOL: Add *Depending on the cell lines used, the lysis volume should be optimized. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. These plates can be read on all HTRF certified readers, thus making their implementation in. culture plate. An. (In the following text, the term YTHDC1 is used frequently as an abbreviation for the m 6 A-recognition domain of YTHDC1). Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The LVis Plate contains 16 spots which can be loaded with 2 microliters of sample for subsequent absorbance measurements. Plate 25 μL of cells in. Introduction. Page 30 4. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Discover the features and benefits of the VICTOR Nivo multimode plate reader, the smallest and most versatile instrument for life science applications. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. VICTOR® Nivo™ multimode plate reader 2 | For research use only. It is typically used to determine the concentration of nucleic acids and proteins. Incubate overnight at RT. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 47 seconds for a 1536-well plate. In addition, stacker. + 4 µL HTRF ® pre-mixed antibo dies 2. For more information about HTRF ® compatible readers and for set-up recommendations, please. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader MLDVM5 SpectraMax® M5e Multi-Mode Microplate Reader (HTRF certified) MLDVM5E To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Cisbio’s 96-well low volume plates can be used with all HTRF assays, for example second messengers, phospho-proteins, cytokines and biomarkers. It measures 96-well plates in less than 20 seconds and 1536-well formats in under one minute. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. Application Area: absorbance, fluorescence, luminescence, and homogeneous time resolved fluorescence assay development. acceptor reagent. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. An HTRF assay measures the fluorescence energy transfer between molecules in close proximity. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. No washing steps are required. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. COMPONENTS ; STORAGE . At its core, Synergy H1 is a monochromator-based multi-mode microplate reader with a quadruple grating architecture for robust performance and flexibility. For more information. FOR HTRF CERTIFIED READER. The CLARIOstar® Plus is our most flexible multi-mode plate reader. High-end TR-FRET/HTRF® performance: certified ®to measure HTRF assays in both black or white plates, the reader guarantees no compromises in any HTRF assay. HTRF KinEASE-TK kit and HTRF 96-well low volume plate were obtained from Cisbio Bioassays. + 4 µL HTRF pre-mixed antibodies . Unlike standard ELISA plate readers, MSD. Our microplates and accessories are manufactured under strict process controls guaranteeing consistent product performance for. FOR HTRF CERTIFIED READER For more information about HTRF. Alpha Technology: The VICTOR Nivo system. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Incubate at room temperature. The optical systems use two scanningHighly compatible plates for HTRF. Read & analyse. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. COMPONENTS ; STORAGE . NON-SPECIFIC BINDING TOTAL BINDING+ 4 µL HTRF pre-mixed antibodies. These plates can be read on all HTRF certified readers, thus making their implementation in. FOR ORE INFORMATION Europe and other ountries 33(0)466 96 0 U. . ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates into a 384-well low volume detection plate before the addition of Total Cereblon HTRF detection reagents. Measure elevated and normal expression samples without multiple dilutions. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Certified HTRF compatible reader by CisBio Bioassays;Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. It measures at any wavelength, with top and bottom reading for most standard technologies. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. To perform the assay, use white plate only. 1. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. pre+ 5 µL HTRFcompounds Activation Buffer. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Lysates are then transferred to the assay plate for the detection of total-STING by HTRF. The multidetection microplate readers CLARIOstar, PHERAstar FS, FLUOstar Omega, and POLARstar Omega are all HTRF-certified by Cisbio, meaning each of the. 68. 3-in-1 absorbance using a variety of sample formats, including microplates. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. For more information about HTRF ®The LUMIstar® Omega is BMG LABTECH's powerful dedicated luminescence microplate reader, providing highly sensitive flash and glow assay readings. 1 – 1. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. The FLUOstar Omega is the ideal plate reader for life science applications. Figure 1. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. To perform the assay, use white plate only. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYONE-PLATE ASSAY PROTOCOL: Small volume detection plate. 5 EXAMPLE OF MAP PLATE . In particular, these parameters should be entered as below. View Brochure. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READERDetection of Total BRD4 with HTRF reagents is performed in a single plate used for plating, stimulation and detection. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. FOR HTRF CERTIFIED READER.